President Recep Tayyip Erdogan on Monday vowed Turkey would remove a Kurdish militia viewed as terrorists by Ankara from northern Syria if the US failed to ensure fighters leave the east of the Euphrates.
The threat comes after Erdogan spoke on the phone with US President Donald Trump on Friday when the pair agreed to ensure "more effective coordination" between their countries' operations in Syria.
The conversation followed threats from Erdogan last week to launch an offensive against the US-backed Kurdish People's Protection Units (YPG) militia east of the Euphrates River, where there are American troops, within the "next few days".
"I spoke with Trump. The terrorists must leave the east of the Euphrates. If they don't go, we will remove them," Erdogan promised during a televised speech in the central Turkish province of Konya.
Turkey views the YPG as a "terrorist offshoot" of the outlawed Kurdistan Workers' Party (PKK), which has waged an insurgency against the Turkish state since 1984.
The PKK is blacklisted as a terror group by Ankara, the US and the European Union.
"They (the YPG) are a source of discomfort with their terror corridor," Erdogan said.
Although Erdogan said that Turkey could start an operation "at any moment", he appeared to indicate there was room for negotiation with the US.
"Since we are strategic partners with America, then we must do what is necessary," said Erdogan. But he added that the US "must fulfil promises" without giving further detail.
The YPG has spearheaded Washington's fight against the Islamic State (IS) extremist group in Syria under the banner of the Kurdish-led Syrian Democratic Forces (SDF).
But US support for the YPG has strained relations between the NATO allies, which worsened recently after the US set up observation posts in northern Syria close to the border with Turkey to prevent any altercation between Turkish forces and the YPG.
Turkey has launched two military operations helping Syrian rebels to recapture territory in Syria from IS and the YPG in August 2016 and January 2018 respectively.
The first offensive in northern Syria lasted until March 2017 targeting IS on the Turkey-Syria border and to stop the YPG gaining more territory.
The second was completed in March 2018 after the Ankara-backed rebels captured the YPG-held enclave of Afrin in northwestern Syria.